UNIT-I
Nuclear Magnetic Resonance spectroscopy
Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical
shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications
Mass Spectrometry-
Principles, Fragmentation, Ionization techniques –Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, application.
UNIT-II
Thermal Methods of Analysis:
Principles, instrumentation and applications
of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)
X-Ray Diffraction Methods:
Origin of X-rays, basic aspects of crystals, X-
ray
Crystallography, rotating crystal technique, single crystal diffraction,powder
diffraction, structural elucidation and applications.
UNIT-III
Calibration and validation-
as per ICH and USFDA guidelines
Calibration of following Instruments
Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer,Fluorimeter, Flame Photometer, HPLC and GC
UNIT-IV
Radio immune assay:
Importance, various components, Principle, different
methods, Limitation and Applications of Radio immuno assay
Extraction techniques:
General principle and procedure involved in the solid
phase extraction and liquid-liquid extraction
UNIT-V
Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS.