PHARMACOLOGICAL SCREENING METHODS

Unit –I 

Laboratory Animals:

Study of CPCSEA and OECD guidelines for maintenance, breeding

and conduct of experiments on laboratory animals, Common lab

animals: Description and applications of different species and strains

of animals. Popular transgenic and mutant animals.

Techniques for collection of blood and common routes of drug

administration in laboratory animals, Techniques of blood collection

and euthanasia.

Unit –II 

Preclinical screening models

a. Introduction: Dose selection, calculation and conversions,

preparation of drug solution/suspensions, grouping of animals and

importance of sham negative and positive control groups.

Rationale for selection of animal species and sex for the study. 

b. Study of screening animal models for

Diuretics, nootropics, anti-Parkinson’s,antiasthmatics,

Preclinical screening models: for CNS activity- analgesic,

antipyretic,anti-inflammatory, general anaesthetics, sedative and

hypnotics, antipsychotic, antidepressant, antiepileptic, antiparkinsonism, alzheimer’s disease

Unit –III

Preclinical screening models: for ANS activity, sympathomimetics,

sympatholytics, parasympathomimetics, parasympatholytics, skeletal

muscle relaxants, drugs acting on eye, local anaethetics

Unit –IV

Preclinical screening models: for CVS activity- antihypertensives,

diuretics, antiarrhythmic, antidyslepidemic, anti aggregatory, coagulants, and anticoagulants

Preclinical screening models for other important drugs like antiulcer,

antidiabetic, anticancer and antiasthmatics.

Research methodology and Bio-statistics

Selection of research topic, review of literature, research hypothesis

and study design

Pre-clinical data analysis and interpretation using Students ‘t’ test

and One-way ANOVA. Graphical representation of data